-
MicroRNAs as Potential Markers Involved in Erythroid Differentiation: A Systematic Literature Review
Chunmei Guo,
Xinli Li,
Shuqing Liu,
Mingzhong Sun
Issue:
Volume 10, Issue 2, June 2021
Pages:
16-29
Received:
6 April 2021
Accepted:
19 April 2021
Published:
23 April 2021
Abstract: As a family of small non-coding RNAs, microRNAs (miRNAs) negatively modulate gene expression via directly targeting mRNAs in a sequence-specific pattern. Accumulated evidences have indicated that miRNAs involved in erythroid differentiation. Some experimental systems used for study the association of miRNAs with erythroid differentiation: 1) embryonic stem cells (hESCs) forced to erythropoiesis, 2) hematopoietic progenitor cells and erythroid-like cell lines induced to erythropoiesis by hypoxia and chemical substances, 3) and in vivo mice, zebrafish embryo systems. Based on the literatures, miR-451, miR-144, miR-486, miR-126-3p, miR-107, miR-199b-5p, miR-362, miR-188, miR-210, miR-125a, miR-146b, miR-22, miR-23a / miR-27a / miR-24, miR-16-2, miR-34a exhibit promotion role in erythropoiesis, while, miR-218, miR-320a, miR-221 / 222, miR-433, miR-200a, miR-223, miR-150, miR-34a-5p, miR-124, miR-Let-7d, miR-376a, miR-155, miR-126 / 126*, miR-103, miR-15a, miR-30a-5p, miR-26a-5p, miR-669m, miR-9 show suppression role in erythropoiesis. Nonetheless, the clear functional role of miR-24 is controversial in erythropoiesis. This article summarized the relationships between miRNAs and erythroid differentiation as well as potential target genes and action mechanisms. These discovered erythroid associated miRNAs stand for the starting point to develop novel approaches for miRNA treatment, miRNAs to be used as novel potential biomarker and target for diagnosis, therapeutics, prognosis of certain blood diseases, leading to promising prospects in blood diseases therapeutics.
Abstract: As a family of small non-coding RNAs, microRNAs (miRNAs) negatively modulate gene expression via directly targeting mRNAs in a sequence-specific pattern. Accumulated evidences have indicated that miRNAs involved in erythroid differentiation. Some experimental systems used for study the association of miRNAs with erythroid differentiation: 1) embryo...
Show More
-
Obesity as a Risk Factor Against COVID-19
Rosa Elvira Minchala Urgilés,
Anabel Estefanía Arce Prieto,
Andrés Alexis Ramírez Coronel,
Lilia Romero Sacoto,
Fanny González León,
María De Los Ángeles Estrella-González,
Pedro Carlos Martínez-Suárez
Issue:
Volume 10, Issue 2, June 2021
Pages:
30-37
Received:
22 April 2021
Accepted:
21 May 2021
Published:
31 May 2021
Abstract: In late 2019, in Wuhan City, China, SARS-CoV2 was identified as the cause of an outbreak of acute respiratory illness called COVID-19 representing its most frequent lethal complication. Objective: To analyze, by means of a literature review, obesity as a risk factor for COVID-19. Methodology: A systematic review of the literature was carried out in scientific databases such as: Latin American and Caribbean Health Sciences Literature (LILACS); Virtual Health Library (VHL): Wiley Online Library and Medical Literature Analysis and Retrieval System Online (MEDLINE), Scielo, Scopus. The search terms included the study variables. Results: According to the analysis of 51 articles, it was determined that obese, male and elderly patients are the profile most prone to present respiratory system failures associated with COVID-19. Their pictures are aggravated by immunological and hormonal physiopathology typical of obesity; in addition, comorbidity in which there is diabetes, hypertension, cardiovascular diseases and obstructive pulmonary disease aggravates the picture of COVID-19 in obese patients. Conclusions: The presence of obesity is indeed a risk factor against COVID-19 infection, since it increases the possibility of suffering severe respiratory conditions.
Abstract: In late 2019, in Wuhan City, China, SARS-CoV2 was identified as the cause of an outbreak of acute respiratory illness called COVID-19 representing its most frequent lethal complication. Objective: To analyze, by means of a literature review, obesity as a risk factor for COVID-19. Methodology: A systematic review of the literature was carried out in...
Show More
-
Creatinine Increase Caused by Etamsylate Interference: A Case Report of Clinical Pharmaceutical Care
Lejia Xu,
Xingling Liu,
Danwei Zheng,
Jiawen Huang
Issue:
Volume 10, Issue 2, June 2021
Pages:
38-41
Received:
13 May 2021
Accepted:
28 May 2021
Published:
3 June 2021
Abstract: Background: The accuracy of test results is closely related to the clinical diagnosis and treatment, while drugs may interfere with the test results. When the abnormal value of results occured, it is important to analyze the drug-related interferential factors. Objective: To remind clinicians and pharmacists to eliminate interference factors when finding abnormal results by recording the cause analysis process of a patient with abnormally elevated creatinine. Methods: In this case of an abnormally elevated creatinine in a patient treated with vancomycin, the clinical pharmacist analyzed the reasons of patients' creatinine elevation, especially vancomycin-associated kidney injury and other interfering factors of combination drugs in the tests. Results: The clinical pharmacist Clinical pharmacists found that acute kidney damage was not caused by vancomycin, the “real killer” was the etamsylate interference factors in laboratory test of creatinine value. This discovery enabled the patient's antibiotic treatment to proceed smoothly. Conclusions: As a clinical pharmacist, it is necessary to understand the medication situation of patients and be familiar with the common types of "drug interference detection". With these advantages in drug knowledge, clinical pharmacists can assist clinicians to identification and judgment of adverse drug reactions, and play an important role in the treatment of patients.
Abstract: Background: The accuracy of test results is closely related to the clinical diagnosis and treatment, while drugs may interfere with the test results. When the abnormal value of results occured, it is important to analyze the drug-related interferential factors. Objective: To remind clinicians and pharmacists to eliminate interference factors when f...
Show More
-
A Case Report: Recurrent Hyperparathyroidism After Parathyroidectomy in a Primary Hyperparathyroidism Patient with Chronic Kidney Disease
Xia Qing Li,
Kai Jun Chen,
Yu Meng
Issue:
Volume 10, Issue 2, June 2021
Pages:
42-46
Received:
18 May 2021
Accepted:
30 May 2021
Published:
10 June 2021
Abstract: Background: Parathyroidectomy (PTX) is the definitive treatment of primary hyperparathyroidism (PHPT) for symptomatic patients. PTX for hyperparathyroidism is associated with the risk of recurrence; however, this has been mostly demonstrated in chronic kidney disease (CKD) patients with secondary hyperparathyroidism. The recurrent risk of hyperparathyroidism and renal outcomes after PTX in patients with PHPT has been rarely reported. Patient: In this article, we report a case of a 70-year-old man with previously diagnosed CKD, underwent a subtotal PTX for asymptomatic primary hyperparathyroidism induced by parathyroid hyperplasias. The PTX appears to resolve the calcium disturbance in this patient. However, at three months after the surgery, the diagnosis of recurrent hyperparathyroidism was made, based on a remarkably increased level of parathyroid hormone from 42.12 pg/ml to 270.9 pg/ml. Over 2 years of follow-up, his renal function, as evaluated by serum creatinine levels, fluctuated modestly, but persist at a preoperative level of renal impairment. These data indicate that this patient with asymptomatic PHPT has experienced recurrent hyperparathyroidism with persistent renal dysfunction after PTX. Conclusion: The recurrent hyperparathyroidism after PTX in PHPT patients with a coexisting renal impairment appears more common than might be anticipated. We recommend that if hyperparathyroidism recurs or exacerbates by the persistent renal dysfunction after PTX, the potential benefits of PTX should be reconsidered and carefully weighed against the possible surgical risks.
Abstract: Background: Parathyroidectomy (PTX) is the definitive treatment of primary hyperparathyroidism (PHPT) for symptomatic patients. PTX for hyperparathyroidism is associated with the risk of recurrence; however, this has been mostly demonstrated in chronic kidney disease (CKD) patients with secondary hyperparathyroidism. The recurrent risk of hyperpara...
Show More
-
The Significance of Urinary RBP and KIM-1 Determination in Cisplatin – Induced Acute Kidney Injury
Yang Haoqiang,
Meng Xiaoyan,
Huang Xiangyang,
Tan Hechang,
Zhang Min,
Shu Tingting
Issue:
Volume 10, Issue 2, June 2021
Pages:
47-51
Received:
6 May 2021
Accepted:
24 May 2021
Published:
25 June 2021
Abstract: Objective: Cisplatin is one of the most commonly used and most effective chemotherapy drugs in clinical treatment of solid tumors, because of its strong anti-cancer effect, no cross resistance with other cancer drugs, but cisplatin is one of the significant renal toxicity drugs either, which greatly limits its clinical application. The traditional diagnostic methods for AKI are to measure Scr and changes in urine volume, but these indicators are affected by non-renal factors and cannot reflect renal function in an early and timely manner, which delays the optimal early treatment time for AKI. To discuss the clinical value of detecting urinary retinol binding protein (RBP) and kidney injury molecule 1 (KIM-1) in early diagonsis of cisplatin-induced acute kidney injury. Methods: 48 rats were randomly divided into the cisplatin group (CP group, 42 rats) and the control group (NS group, 6 rats). The urinary RBP and KIM-1, renal pathological changes were observed at each injection time points. Results: Urinary KIM-1 it significantly increased at 12h point (P<0.05), which was higher obviously than NS group. Urinary RBP it significantly elevated at 6h point (P<0.05). It has correlation with Scr. Conclusion: In cisplatin-induced acute kidney injury, urinary RBP and KIM-1 increased obviously earlier than serum creatinine, they were early sensitive markers.
Abstract: Objective: Cisplatin is one of the most commonly used and most effective chemotherapy drugs in clinical treatment of solid tumors, because of its strong anti-cancer effect, no cross resistance with other cancer drugs, but cisplatin is one of the significant renal toxicity drugs either, which greatly limits its clinical application. The traditional ...
Show More
-
Identification of Functional Gene Modules and Biomarkers of Apatinib Against Lung Adenocarcinoma Based on Weighted Gene Co-expression Network Ansalysis (WGCNA)
Zhang Yanli,
Luo Shiqiong,
Zhong Yixuan,
Wu Guosong,
Zhang Zhidong
Issue:
Volume 10, Issue 2, June 2021
Pages:
52-64
Received:
10 June 2021
Accepted:
19 June 2021
Published:
25 June 2021
Abstract: Objective: Apatinib is a drug for the treatment of gastric cancer. In recent years, studies have found that it also has good efficacy in the treatment of lung adenocarcinoma, but its mechanism of action is still not clear. Therefore, this study uses bioinformatics and Experimental verification explores the mechanism of apatinib in the treatment of lung adenocarcinoma. To explore the potential biological targets of apatinib against lung adenocarcinoma based on weighted gene co-expression network analysis (WGCNA). Methods: The PharmMapper server was first used to reversely predict the potential targets of apatinib. Subsequently, it was combined with WGCNA to mine the GSE10072 data set in the GEO database of the National Center for Biotechnology Information (NCBI) to obtain the co-expressed gene module. Next, it was combined with apatinib Predict the target matching mapping to obtain the potential anti-lung adenocarcinoma target of apatinib. The STRING database was combined with Cytoscape software to visualize the protein interaction network of apatinib's potential anti-lung adenocarcinoma target protein and perform network topology analysis, as well as to obtain the core target from the network. Kaplan Meier plotter database was applied to analyze the relationship between key genes and the prognosis of patients with lung adenocarcinoma. Molecular docking technology was used for the potential of apatinib. Anti-lung adenocarcinoma target protein was finally verified by molecular interaction. Finally, Western blot was employed to analyze the expression level of the key target protein. Results: A total of 300 targets of apatinib were obtained, and 50 potential targets of apatinib against lung adenocarcinoma were screened. From the protein interaction network analysis, the key genes included CASP3, EGFR, MMP9, SRC, CASP8, CASP9, STAT3 and MAPK1. High expression of EGFR, SRC, MAPK1 and STAT3, and low expression of CASP3, CASP8, CASP9 and MMP9 were closely related to poor prognosis of patients with lung adenocarcinoma. Molecular docking showed that the interaction of apatinib with the targets of MAPK1, CASP3, EGFR, SRC, MMP9 and STAT3 was comparable to the positive control. Western blot showed that with the increasing of drug concentration, STAT3 were down-regulated, and CASP8 were up-regulated. Conclusion: The mechanism of apatinib in the treatment of lung adenocarcinoma is mainly through the regulation of the signal transduction pathway and the apoptosis pathway, which provides a scientific basis for the study of its anti-lung adenocarcinoma mechanism.
Abstract: Objective: Apatinib is a drug for the treatment of gastric cancer. In recent years, studies have found that it also has good efficacy in the treatment of lung adenocarcinoma, but its mechanism of action is still not clear. Therefore, this study uses bioinformatics and Experimental verification explores the mechanism of apatinib in the treatment of ...
Show More
-
The Profile of COVID-19 Exposure in Healthcare Workers in Department of Medical Rehabilitation National General Hospital Cipto Mangunkusumo
Luh Karunia Wahyuni,
Widjajalaksmi Kusumaningsih,
Melinda Harini,
Steven Setiono,
Rizky Kusuma Wardhani,
Peggy Sunarjo,
Budiati Laksmitasari
Issue:
Volume 10, Issue 2, June 2021
Pages:
65-71
Received:
8 June 2021
Accepted:
21 June 2021
Published:
26 June 2021
Abstract: Background: Healthcare workers are a population groups at high risk of contracted by COVID-19 and some of the rehabilitation team involved in some therapeutic measures that produce the aerosol that further increase the chances of exposure to COVID-19. This study aims to assess the profile of COVID-19 exposure in healthcare worker in medical rehabilitation department National General Hospital Cipto Mangunkusumo. Methods: The study has been done in August 2020 to February 2021. The research subjects were all the healthcare workers who have met the inclusion and exclusion criteria. The data was collected from the questionnaires. Results: There were 289 cases involved, mostly women (74.4%) and the resident doctors (59.2%). The average age was 36 years. The subjects who had comorbidities were 41.2%. The highest source of exposure came was the interaction among the healthcare workers (85.1%) in the locations that they were usually gathered (65.4%). The suitability of protective personal equipment only 70.6%. Conclusion: The profile of COVID-19 exposure in healthcare worker among medical rehabilitation team in this hospital was dominated by young people, women, resident doctors, and without comorbidities. The pattern of the transmission dominated by interaction among the healthcare workers in area where they were gathered. It is hoped that this study can become the consideration in making policy regarding the transmission prevention among healthcare workers.
Abstract: Background: Healthcare workers are a population groups at high risk of contracted by COVID-19 and some of the rehabilitation team involved in some therapeutic measures that produce the aerosol that further increase the chances of exposure to COVID-19. This study aims to assess the profile of COVID-19 exposure in healthcare worker in medical rehabil...
Show More
